Source code for jii_multispeq_protocols.protocols.calibrations.fluorescence_detector_offsets_calibration
"""
Fluorescence - Detector Offsets
===============================
allows for correction of small offsets in the measurements caused by actinic light current/ground loop on main and addon boards
Used with Bleed CAL#7B
These calibration steps require the chlorophyll calibration and the pink fluorescent cards
2231
"""
import numpy as np
from scipy import stats
import warnings
from jii_multispeq.analysis import GetProtocolByLabel
_protocol = [
{
"v_arrays": [
[
0,
-200,
-400,
-750,
-1000,
-2000,
-3000,
-4000
],
[
200,
30
],
[
2,
9
],
[
1,
0,
1,
0
]
],
"set_repeats": "#l2",
"_protocol_set_": [
{
"alert": "Clamp the Fluorescent Card (pink)",
"do_once": 1,
"autogain": [
[
1,
1,
3,
15,
50000
],
[
6,
6,
1,
15,
50000
],
[
0,
10,
1,
15,
50000
]
]
},
{
"label": "bc1",
"bleed_correction": 1,
"pre_illumination": [
2,
0,
"@n1:0"
],
"pulses": [
"@n1:1",
"@n1:1",
"@n1:1"
],
"pulses_delay": [
1,
1,
1
],
"nonpulsed_lights": [
[
"@s2"
],
[
"@s2"
],
[
"@s2"
]
],
"pulse_distance": [
2000,
2000,
2000
],
"nonpulsed_lights_brightness": [
[
0
],
[
"@p0"
],
[
0
]
],
"pulsed_lights": [
[
1,
6,
10,
3
],
[
1,
6,
10,
3
],
[
1,
6,
10,
3
]
],
"detectors": [
[
3,
1,
1,
1
],
[
3,
1,
1,
1
],
[
3,
1,
1,
1
]
],
"pulsed_lights_brightness": [
[
"a_b1",
"a_b6",
"a_b0",
400
],
[
"a_b1",
"a_b6",
"a_b0",
400
],
[
"a_b1",
"a_b6",
"a_b0",
400
]
],
"pulse_length": [
[
"a_d1",
"a_d6",
"a_d0",
20
],
[
"a_d1",
"a_d6",
"a_d0",
20
],
[
"a_d1",
"a_d6",
"a_d0",
20
]
],
"protocol_averages": 1,
"protocol_repeats": "#l0"
},
{
"label": "bc0",
"bleed_correction": 0,
"pre_illumination": [
2,
0,
"@n1:0"
],
"pulses": [
"@n1:1",
"@n1:1",
"@n1:1"
],
"pulses_delay": [
1,
1,
1
],
"nonpulsed_lights": [
[
"@s2"
],
[
"@s2"
],
[
"@s2"
]
],
"pulse_distance": [
2000,
2000,
2000
],
"nonpulsed_lights_brightness": [
[
0
],
[
"@p0"
],
[
0
]
],
"pulsed_lights": [
[
1,
6,
10,
3
],
[
1,
6,
10,
3
],
[
1,
6,
10,
3
]
],
"detectors": [
[
3,
1,
1,
1
],
[
3,
1,
1,
1
],
[
3,
1,
1,
1
]
],
"pulsed_lights_brightness": [
[
"a_b1",
"a_b6",
"a_b0",
400
],
[
"a_b1",
"a_b6",
"a_b0",
400
],
[
"a_b1",
"a_b6",
"a_b0",
400
]
],
"pulse_length": [
[
"a_d1",
"a_d6",
"a_d0",
20
],
[
"a_d1",
"a_d6",
"a_d0",
20
],
[
"a_d1",
"a_d6",
"a_d0",
20
]
],
"protocol_averages": 1,
"protocol_repeats": "#l0"
}
]
}
]
[docs]
def _analyze( _data ):
"""
Macro for data evaluation artifacts on measurements caused by actinic light ground loops
data on PhotosynQ.org
by: David M. Kramer
created: 2017-05-09 @ 18:15:27
"""
## Define the output object here
graphs = False
output = {} ## dictionary to hold results
n_sets = len(_data["set"])
# {\"Light 1\":\"530nm, green, main\"\
# ,\"Light 2\":\"655nm red, main)\"\
# ,\"Light 3\":\"590nm amber, main)\"\
# ,\"Light 4\":\"448nm blue, main)\"\
# ,\"Light 5\":\"950nm NIR, main)\"\
# ,\"Light 6\":\"950nm NIR, clamp)\"\
# ,\"Light 7\":\"655 red, clamp\"\
# ,\"Light 8\":\"850nm NIR, clamp\"\
# ,\"Light 9\":\"730nm far red, clamp\"\
# ,\"Light 10\":\"810nm NIR, clamp\"\
# ,\"Detector 1\":\"700nm - 1150nm, main\"\
# ,\"Detector 3\":\"400nm - 700nm, clamp\"\
# ,\"Ambient Temperature, Humidity, Pressure\":\"thp\"\
# ,\"Ambient Temperature, Humidity, Pressure 2\":\"thp2\"\
# ,\"Contactless Temperature\":\"contactless_temp\"\
# ,\"Accelerometer\":\"compass_and_angle\"\
# ,\"Magnetometer\":\"compass_and_angle\"\
# ,\"Hall Effect Sensor\":\"thickness\"\
# ,\"PAR light sensor\":\"light_intensity\"}"
# FOR actinic LED #2
# Assuming we use LED#2 as actinic
# led# det1 det3
# 1 2 1
# 2 9 9 # 9 means use some generic correction
# 3 2 1
# 4 2 1
# 5 2 1
# 6 3 9
# 7 4 9
# 8 3 9
# 9 5 9
# 10 6 9
bleedArrays = {}
MapLedToDet = [[2,9,2,2,2,3,4,5,6,7],[1,9,1,1,1,9,9,9,9,9]] ## the first dimension is the det#, the second is the LED#
bleedArrays[2]= MapLedToDet
# FOR actinic LED #9
# Assuming we use LED#2 as actinic
# led# det1 det3
# 1 0 1
# 2 9 9 # 9 means use some generic correction
# 3 2 1
# 4 2 1
# 5 2 1
# 6 3 9
# 7 4 9
# 8 3 9
# 9 5 9
# 10 6 9
MapLedToDet_act9 = [[8,9,8,8,9,10,11,12,9,13],[14,9,14,14,14,9,9,9,9,9]] ## the first dimension is the det#, the second is the LED#
bleedArrays[9]= MapLedToDet_act9
x = _data["set"][0]
bleed_correction = [1,0]
Extr = []
## set upper limits (+/1) for testing of each LED with each actinic light
Xbounds_for_act_2 = [[30,20,20,20,20,30,20,20,30],[20,20,20,20,20,30,20,20,20,30]]
Extr.append(Xbounds_for_act_2)
Xbounds_for_act_9 = [[30,30,30,30,30,30,20,20,20,30],[50,20,30,20,20,30,20,20,20,30]]
Extr.append(Xbounds_for_act_9)
act_settings = _data["v_arrays"][0]
for i in range(len(act_settings)): ## if direct DAC setting, mulitple by -1
if act_settings[i] < 0:
act_settings[i] = -1 * act_settings[i]
mLEDs = [1,6,10,3]
mDetectors = [1,0,0,0]
aLEDs = _data["v_arrays"][2]
nMeasuringLEDS = len(mLEDs)
mLedNames = {1:"530nm", 2:"655nm", 3:"590nm", 4:"488nm", 5:"940nm", 6:"b940nm", 7:"b655nm", 8:"b850nm", 9:"b730nm", 10:"b810nm"}
nSettings = len(act_settings)
toDevice=""
for i in range(15):
toDevice += "bleed3+%s+0+0+0+0+0+0+0+0+" % i
issues = 0
for aLEDindex in range(len(aLEDs)):
actBleed = []
b = nSettings * aLEDindex
e = nSettings * (1 + aLEDindex)
t = GetProtocolByLabel( "bc0", _data, True )[b:e]
actBleed.append(t)
# output["trace_%s" % aLEDindex] = t["data_raw"] #TODO: This is not working, not sure why it is in the js code like this
t = GetProtocolByLabel( "bc1", _data, True )[b:e]
actBleed.append(t)
actBleedTrace = {}
subPoints = _data["v_arrays"][1][1]
LEDs = _data["v_arrays"][2] ## set up array for LEDs tested. Note: this does not include the baseline
for abi in range(len(actBleed)): ## cycle through the repeats of the entire experiment, each with different actinic light intensities.
tname = []
offset = {}
for ledidx in range(len(mLEDs)):
tn = "actBleed_%s_aLED_%s_det_%s_bleed_%s" % (mLedNames[mLEDs[ledidx]], aLEDs[aLEDindex], mDetectors[ledidx], actBleed[abi][0]["bleed_correction"])
tname.append(tn)
actBleedTrace[tn] = []
offset[tn] = []
actbleed = actBleed[abi] ## set actbleed to the array of traces in this run of the set
for ledidx in range(len(mLEDs)):
numIntensities = len(actbleed)
for i in range(numIntensities): ## extract traces for each detector, subtract the baseline
t = actbleed[i]["data_raw"][ledidx::nMeasuringLEDS]
tm = np.mean(t[0:subPoints])
t = np.array(t) - tm ## subtract off the baseline
actBleedTrace[tname[ledidx]].append(t)
averageOffset = np.mean(t[int(subPoints)+3 : 2*subPoints])
offset[tname[ledidx]].append(averageOffset)
# if actbleed[i]["bleed_correction"] == 1: #TODO: Seems like it is not used
# Xbounds = Extr[aLEDindex]
# b = Xbounds[aLEDindex][mLEDs[ledidx]-1]
if graphs == True:
output["offset_%s" %tname[ledidx]] = offset[tname[ledidx]]
ledN = mLEDs[ledidx]
detN = mDetectors[ledidx]
bleedArray = bleedArrays[aLEDs[aLEDindex]]
if actbleed[0]["bleed_correction"] == 0:
toDevice+="bleed3+%s+" % bleedArray[detN][ledN-1] ## MapLedToDet[mDetectors[ledidx]-1][mLEDs[ledidx]-1];
for j in range(8):
toDevice += "%s+" % np.round(10 * offset[tname[ledidx]][j], 0)
output["toDevice"] = toDevice
if issues == 0:
output["status"] = " Test OK"
else:
output["status"] = " WARNING: %s found! Try sending calibration data to instrument." % issues
output["toDevice"] = toDevice
output["toDevice"] += "setCalTime+2+setCalOK+2+0+"
return output
_example = {
"time": 1600040142460,
"device_name": "MultispeQ",
"device_version": "2",
"device_id": "52:00:12:bc",
"device_battery": 98,
"device_firmware": 2.343,
"sample": [
[
{
"time": 1600040142460,
"v_arrays": [
[
0,
-200,
-400,
-750,
-1000,
-2000,
-3000,
-4000
],
[
200,
30
],
[
2,
9
],
[
1,
0,
1,
0
]
],
"set_repeats": 2,
"protocol_id": 1,
"set": [
{
"time": 1600040142475,
"message": [
"alert",
"Clamp the Fluorescent Card (pink)",
"ok"
],
"autogain": [
[
1,
1,
3,
15,
-4294,
29562
],
[
6,
6,
1,
15,
-1342,
27708
],
[
0,
10,
1,
15,
-1095,
45919
]
],
"data_raw": [
]
},
{
"time": 1600040150908,
"bleed_correction": 1,
"label": "bc1",
"data_raw": [
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},
{
"time": 1600040152037,
"bleed_correction": 1,
"label": "bc1",
"data_raw": [
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},
{
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"bleed_correction": 1,
"label": "bc1",
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