Photosynthesis (RIDES)

Measures the same photosynthesis-related parameters in <15 seconds as ‘Leaf Photosynthesis MutlispeQ v2.0’ plus additional PS1 and P700 measurements.

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF

  • Relative Chlorophyll

  • ATP synthase conductivity, proton motive force parameters through DIRK(ECS): ECSt mAU, vH+, gH+

  • Saturation pulse estimation of PSI parameters: PS1 active centers, PS1 open, PS1 oxidized and PS1 over reduced

  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.

  • The rate of turnover of the cytochrome b6f complex through DIRKP(700)

  • Leaf Thickness (in mm), angle, and cardinal direction

  • Leaf Temperature and differential from ambient temperature

  • Environmental conditions: PAR and ambient temperature/pressure/humidity

Important

Supports MultispeQ V1 and V2 with Firmware 2.34 and higher

Import

Example: Import rides into a script
import jii_multispeq_protocols.rides as _rides

Protocol

[{'_protocol_set_': [{'averages': 1,
                   'energy_min_wake_time': 7000,
                   'energy_save_timeout': 300000,
                   'environmental': [['light_intensity'],
                                     ['temperature_humidity_pressure'],
                                     ['temperature_humidity_pressure2'],
                                     ['contactless_temp'],
                                     ['compass_and_angle']],
                   'label': 'no_leaf_baseline',
                   'par_led_start_on_open': 2},
                  {'autogain': [[2, 1, 3, 12, 50000], [3, 8, 1, 80, 50000]],
                   'averages': 1,
                   'detectors': [[3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [3],
                                 [1],
                                 [1],
                                 [1]],
                   'environmental': [['light_intensity'],
                                     ['temperature_humidity_pressure'],
                                     ['temperature_humidity_pressure2'],
                                     ['contactless_temp'],
                                     ['thickness']],
                   'label': 'DIRK_ECS',
                   'nonpulsed_lights': [[2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2]],
                   'nonpulsed_lights_brightness': [['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity']],
                   'par_led_start_on_close': 2,
                   'protocols_delay': 10,
                   'pulse_distance': [1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500],
                   'pulse_length': [['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2'],
                                    ['a_d2']],
                   'pulsed_lights': [[1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [1],
                                     [3],
                                     [3],
                                     [3]],
                   'pulsed_lights_brightness': [['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2'],
                                                ['a_b2']],
                   'pulses': [100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              20,
                              20,
                              100]},
                  {'averages': 1,
                   'detectors': [[1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1],
                                 [1]],
                   'environmental': [['previous_light_intensity'],
                                     ['temperature_humidity_pressure'],
                                     ['temperature_humidity_pressure2'],
                                     ['contactless_temp'],
                                     ['compass_and_angle']],
                   'label': 'DIRK_P700',
                   'nonpulsed_lights': [[2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2]],
                   'nonpulsed_lights_brightness': [['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity'],
                                                   ['light_intensity'],
                                                   [0],
                                                   ['light_intensity']],
                   'protocols_delay': 1,
                   'pulse_distance': [1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500,
                                      1500],
                   'pulse_length': [['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3'],
                                    ['a_d3']],
                   'pulsed_lights': [[8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8],
                                     [8]],
                   'pulsed_lights_brightness': [['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3'],
                                                ['a_b3']],
                   'pulses': [100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100,
                              100,
                              20,
                              100]},
                  {'averages': 1,
                   'detectors': [[1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [0],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1],
                                 [1, 1]],
                   'environmental': [['previous_light_intensity'],
                                     ['temperature_humidity_pressure'],
                                     ['temperature_humidity_pressure2'],
                                     ['contactless_temp'],
                                     ['thickness'],
                                     ['compass_and_angle']],
                   'label': 'PAM',
                   'nonpulsed_lights': [[2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [9],
                                        [0],
                                        [2],
                                        [2],
                                        [2],
                                        [2],
                                        [2]],
                   'nonpulsed_lights_brightness': [['light_intensity'],
                                                   [8000],
                                                   [7000],
                                                   [6000],
                                                   [5000],
                                                   [4000],
                                                   [0],
                                                   [2090],
                                                   [0],
                                                   [8000],
                                                   [6000],
                                                   [4000],
                                                   [3000],
                                                   ['light_intensity']],
                   'pulse_distance': [5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000,
                                      5000],
                   'pulse_length': [[30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [0],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3'],
                                    [30, 'a_d3']],
                   'pulsed_lights': [[3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [0],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8],
                                     [3, 8]],
                   'pulsed_lights_brightness': [[400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [0],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3'],
                                                [400, 'auto_bright3']],
                   'pulses': [100,
                              30,
                              15,
                              15,
                              15,
                              15,
                              15,
                              600,
                              15,
                              30,
                              15,
                              15,
                              15,
                              15]},
                  {'averages': 1,
                   'environmental': [['light_intensity'],
                                     ['temperature_humidity_pressure'],
                                     ['temperature_humidity_pressure2'],
                                     ['contactless_temp'],
                                     ['thickness'],
                                     ['compass_and_angle']],
                   'label': 'SPAD',
                   'spad': [1]}]}]

Analysis Function

_analyze(_data)[source]

Data evaluation of RIDES

by: David M. Kramer created: 2017-05-09 @ 18:15:27

Analysis Example (requires JII-MultispeQ package)
from jii_multispeq import measurement

## Take a measurement using the MultispeQ
data, crc32 = jii_multispeq.measure(port="<Selected Port>", protocol=_rides, filename=None, notes="")

## The analyze function of JII-MultispeQ helps to provide the correct format
output = measurement.analyze( data, _rides._analzye )

## View Analysis output (as table)
measurement.view( output )