Relative Chlorophyll Content (SPAD)¶

This protocol measures basic absorbance at 650 nm and 940 nm and calculates the relative chlorophyll content.

The value returned represents the Special Products Analysis Division (SPAD) to make it comparable to other instruments used in the field.

\(SPAD = k \times \log_{10}\frac{Abs_{940nm} / ref. Abs_{940}}{Abs_{650nm} / ref. Abs_{650}}\)

\(k\) is a proprietary correlation coefficient used in the Minolta’s calculation of SPAD.

Note

Only works with MultispeQ v2.0

Import

Example: Import spad into a script¶

import jii_multispeq_protocols.spad as _spad

Protocol

Warning

Protocol failed automated validation!

Path ‘0 -> _protocol_set_ -> 0 -> spad’: [[2, 3, 6], [-1]] is not valid under any of the given schemas

[{'_protocol_set_': [{'label': 'spad',
                   'protocol_repeats': 1,
                   'spad': [[2, 3, 6], [-1]]}]}]

Analysis Function

_analyze(data)[source]: Macro for data evaluation on PhotosynQ.org by: David M. Kramer created: 2017-06-21 @ 10:36:29

Analysis Example (requires JII-MultispeQ package)¶

from jii_multispeq import measurement

## Take a measurement using the MultispeQ
data, crc32 = jii_multispeq.measure(port="<Selected Port>", protocol=_spad, filename=None, notes="")

## The analyze function of JII-MultispeQ helps to provide the correct format
output = measurement.analyze( data, _spad._analzye )

## View Analysis output (as table)
measurement.view( output )