Relative Chlorophyll Content (SPAD)¶

This protocol measures basic absorbance at 650 nm and 940 nm and calculates the relative chlorophyll content.

The value returned represents the Special Products Analysis Division (SPAD) to make it comparable to other instruments used in the field.

\(SPAD = k \times \log_{10}\frac{Abs_{940nm} / ref. Abs_{940}}{Abs_{650nm} / ref. Abs_{650}}\)

\(k\) is a proprietary correlation coefficient used in the Minolta’s calculation of SPAD.

Note

Only works with MultispeQ v2.0

Basic Usage¶

Example: Import statement for the spad protocol¶

## Import spad
from jii_multispeq_protocols.protocols import spad as _spad

Sequence¶

        %%{
init: {
  "theme": "base",
  "themeVariables": {"primaryColor": "#49e06d", "primaryTextColor": "#005e5e", "primaryBorderColor": "#005e5e", "lineColor": "#fff381", "secondaryColor": "#afd7f4", "tertiaryColor": "#D7EBF9"}
}
}%%
flowchart LR
     START((Start))
     A0["`**spad**`"]:::protocol
     START ==> A0
     A0 ==> END
     END((&nbsp;End&nbsp;))
     classDef protocol text-align:left,white-space:pre;

Details¶

Code¶

Protocol Code¶

[  {  '_protocol_set_': [  {  'label': 'spad',
                              'protocol_repeats': 1,
                              'spad': [[2, 3, 6], [-1]]}]}]

Warning

Protocol failed automated validation!

Path ‘0 -> _protocol_set_ -> 0 -> spad’: [[2, 3, 6], [-1]] is not valid under any of the given schemas

Analysis¶

Usage¶

Analysis Example (requires JII-MultispeQ package)¶

from jii_multispeq import measurement as _measurement
from jii_multispeq_protocols.protocols import spad as _spad

## Take a measurement using the MultispeQ
data, crc32 = _measurement.measure(port="<Selected Port>", protocol=_spad, filename=None, notes="")

## The analyze function of JII-MultispeQ helps to provide the correct format
output = _measurement.analyze( data, _spad._analyze )

## View Analysis output (as table)
_measurement.view( output )

Function Details¶

_analyze(data)[source]: Macro for data evaluation on PhotosynQ.org by: David M. Kramer created: 2017-06-21 @ 10:36:29

Note

No MultispeQ output example found, missing _example, check manually.